Figure 4.

Anti-CXCR4 antibody and SDF-1 inhibit engraftment. (A, B, C) Cryosections from mouse muscle injected with 1 × 104 freshly isolated mixed canine muscle-derived mononuclear cells alone, cells pre-incubated with anti-CXCR4, cells pre-incubated with control antibody, or CXCR4-positive sorted cells, were immunostained with anti-dystrophin (MANDYS107) or anti-lamin A/C, and fluorescently labeled secondary antibody. The number of fibers expressing canine dystrophin (A), the number of nuclei expressing canine lamin A/C (B), and the number of nuclei expressing canine lamin A/C and Pax7 (C) per cross-section were determined. The bars represent the average of the averages ± SD, where the average was calculated from three cryosections per mouse, and the average of the averages was calculated from three mice per condition. The P values are the result of a Student's t-test. (D, E, F) Skeletal muscle cryosections from NOD/SCID mouse muscle injected with 1 × 104 canine muscle-derived mononuclear cells alone, or cells incubated with 10 ng/ml SDF-1 or FGF-2 before injection, were immunostained with anti-dystrophin (MANDYS107) or anti-lamin A/C, and fluorescently labeled secondary antibody. The number of fibers expressing canine dystrophin (D), the number of nuclei expressing canine lamin A/C (E), and the number of nuclei expressing canine lamin A/C and Pax7 (F) per cross-section were determined. The bars represent the average of the averages ± SD, where the average was calculated from three cryosections per mouse, and the average of the averages was calculated from three mice per condition. The P values are the result of a Student's t-test.

Parker et al. Skeletal Muscle 2012 2:4   doi:10.1186/2044-5040-2-4
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