Figure 2.

Dynamics of T-tubule vacuolation produced by the efflux of glycerol. A single frog skeletal muscle fiber stained with a lipophilic fluorescence probe is shown. Shown are serial confocal microscopic images of the same fiber (a) 5 minutes, (b) 12 minutes and (c) 30 minutes after the fiber was transferred from a solution containing 110 mM glycerol to a solution without glycerol (reprinted from [10]; ^© 2001 with permission from Elsevier).