Chromatin immunoprecipitation (ChIP) analysis of the leiomodin 2 (Lmod2) promoter. Cross-linked extracts from proliferating myoblasts (UD, undifferentiated cells) and myofibers in differentiation media for the indicated number of days (D1-D10) were immunoprecipitated with antibodies against myogenin, MyoD, Myf5, HEB, RNA polymerase II (RNAP II), histone H3 acetylated at lysine 9 and/or 18 (H3 Ac9/18) or IgG. Immunoprecipitated DNA was purified and amplified with primers specific to the promoter of Lmod2. Relative enrichment at the IgH locus was used to normalize the data. The fold enrichment values were calculated as described in Methods.
Londhe and Davie Skeletal Muscle 2011 1:14 doi:10.1186/2044-5040-1-14