Expression of myogenic regulatory factors (MRFs) and E proteins over a time course of differentiation in C2C12 cells. C2C12 cells were differentiated for the indicated number of days and harvested for protein (UD, undifferentiated cells; D1-D10, cells differentiated for the indicated number of days). Protein concentration was determined for each extract and used to normalize the sample loading. Parallel blots were probed for each of the indicated antibodies as described in Methods.
Londhe and Davie Skeletal Muscle 2011 1:14 doi:10.1186/2044-5040-1-14